There are several different types of E. coli bacteria, and o…

There are several different types of E. coli bacteria, and only some of them cause disease in humans. Some of the disease-causing types produce a toxin (a protein) called Shiga toxin. The figure below is related to clinical samples of E. coli. “PCR for the Shiga toxin gene was performed on E. coli samples from healthy volunteers and patients with bloody diarrhea. Purified DNA from a known Shiga toxin-positive E. coli isolate was used in the positive control reaction (+), and the negative control reaction contained no DNA (-).” What kind of experimental technique was used?