Blog

How can all of the mRNA within a sample be amplified to prepare microarray probes?

What is the unique characteristics of the DNA polymerase, Taq DNA polymerase, used in PCR?

Which method has been used successfully to reduce contamination in the preamplification stage of PCR?

A FISH test is performed on a slide of peripheral blood leukocytes. The test uses a dual fusion probe, consisting of a Spectrum Green-labeled probe to the BCR 22 q11.2 locus, and a Spectrum Orange-labeled probe to ABL 9q34. What disease is this test for?

Refer to the figure. An amplicon for gene X is 176 base pairs (bp) in size. A variant allele x, gives the same size amplicon but has an Alwl restriction enzyme recognition site that is not present in the amplicon arising from allele X. A PCR reaction is run on a DNA sample isolated from 3 patients. The sample is digested with Alwl after the PCR is complete and products of the digestion are resolved on a 4.0% agarose gel.Assuming all appropriate controls have worked correctly, what is the interpretation of the genotype of patient #3 with regards to alleles X and x?

Which statement best describes the method of branched DNA signal amplification?

Probes are often used during real time PCR to quantitate the formation of specific amplicons during the reaction. Which system involves the use of 2 distinct probes to generate a fluorescent signal?

What gene must be amplified in PCR to differentiate methicillin-resistant Staphylococus aureus from methicillin-resistant coagulase-negative Staphylococus?

_______ is the start codon for all polypeptides (regardless of their function) and codes for the insertion of _________ within a polypeptide.

The promoter is the binding site for RNA polymerase. It usually lies 5’ to, or upstream of the transcription start site.