RNA Polymerase was shown biochemically to bind tightly to th…

RNA Polymerase was shown biochemically to bind tightly to the following 50 nucleotide piece of double-stranded DNA from the E. coli genome, indicating that it likely contains a basal prokaryotic promoter.  The -10 promoter recognition sequence is underlined. Star indicates the nucleotide pair that occurs at the +1 transcription start site. Given the double-stranded sequence below, answer the following questions:                        Please watch your spelling!    a) Based on your analysis of this promoter region, which strand, top or bottom, will be the template strand? Template will be [tmpl] strand   b) Based on your analysis of this promoter region, in which direction, left or right, will transcription proceed?Transcription will move to the [drc]   c) Write the first eight nucleotides that will make up the beginning of the mRNA made from this promoter:The first 8 nucleotides will be: 5’ [sequence]