You work in a lab dedicated to characterizing the physiology…

You work in a lab dedicated to characterizing the physiology of a mammalian ligand-gated potassium channel (functions as an oligomer, ligand is PIP2). The channel has several post-translational modifications that regulate stability and function. You decide you want to solve the structure. 1. (4pts total) Before you solve the structure, you want to understand the protein topology. The sequence of the protomer has two membrane-spanning transmembrane helices. With the below sequence, please enclose likely TM domains in [brackets] (2pt). Also, please enclose loop(s)/tail(s) that are likely cytoplasmic facing in (parentheses) (2pts).  MKKSRVRKYA SVAVITLVPM LSFAIFWEGD DPAKAAFNSL QASATEYIGYAWAMVVVIVG ATIIYKLFKK FTSKAS   2. (2pts) Another lab found a well-behaved protein with similar physiological function in Aspergillus fumigatus (a fungus) that has 42% sequence identity. Would solving the structure of this protein likely inform understanding of the structure of mammalian protein? Why or why not? 3. (8pts total) Propose a workflow to generate the purified mammalian channel (4pts, 1pt per step), provide a specific example and your rationale at each step. How could you determine if the purified protein is likely in the native state (2pt)? Describe two ways you could try to optimize potential issues in your purification workflow (such as instability, impure protein, misfolding) (2pt).  

Which parenting style has parents set rules and explain the…

Which parenting style has parents set rules and explain the reasons behind them? They are also flexible and willing to make exceptions to the rules in certain cases—for example, temporarily relaxing bedtime rules to allow for a nighttime swim during a family vacation. Of the four parenting styles, this style is the one that is most encouraged in modern American society.