You are working with Neurospora, a fungus that generates an…

Questions

Yоu аre wоrking with Neurоsporа, а fungus that generates an ascus with 8 ordered spores. You create a heterozygous diploid strain by mating a parent of the genotype of Leu+ AmpR Ade+ with another parent of the genotype Leu- AmpS Ade-. All 3 of these genes are linked in the order written. Leu+ allows cells to synthesize leucine, which is an amino acid that is necessary for the fungus to live, Leu- cells cannot synthesize leucine. AmpR confers resistance to ampicillin, AmpS cells are sensitive to ampicillin. Ade+ cells turn red when grown in appropriate conditions, while Ade- cells are white. You induce meiosis in your diploid strain and analyze the individual spores of the resulting asci. Among your population of asci, you find one ascus with spores of the phenotypes listed in the table below. The spores are numbered in the order they were positioned in the ascus. Spores Leucine Ampicillin Color Genotype 1 Prototroph Resistant Red [1] 2 Prototroph Resistant Red [2] 3 Prototroph Resistant White [3] 4 Prototroph Sensitive White [4] 5 Auxotroph Sensitive Red [5] 6 Auxotroph Sensitive Red [6] 7 Auxotroph Sensitive White [7] 8 Auxotroph Sensitive White [8] A. Write the full genotype of each spore into the genotype column of the table. Use the format in the first paragraph above for this (Leu+ or Leu-, AmpR or AmpS, and Ade+ or Ade-) B. What is the aberrant spore genotype ratio in this ascus? [ratio] (please indicate both the ratio and the gene affected) C. Briefly describe the different ways this aberrant ratio could have arisen. You only need to include 2 things here: --what kind(s) of heteroduplex(es) were possible --how the heteroduplex(es) were repaired and what they were repaired to, if at all  [how]