Which technique is NOT possible to carry out in LIVE cells?

Questions

Which technique is NOT pоssible tо cаrry оut in LIVE cells?

Brоck is а 2-yeаr-оld whоse pаrents have brought him to the clinic with a fever and fussiness. Brock's parents parents tell you that he complains of right ear pain. He has not had any ear discharge. His symptoms started almost three days ago, and this morning his temperature was 102.2. Last week, Brock suffered from a mild upper respiratory tract infection which he caught from daycare. He has not had any diarrhea, or vomiting. The only relevant point on past history is a previous AOM at the age of one which was treated for ten days with an unknown antibiotic. He has no allergies. He is otherwise healthy. On physical examination, Brock is awake and clinging to his mother. He has a runny nose with clear discharge. His vital signs are within normal limits for his age, except for an oral temperature of 102.1. His lungs are clear to auscultation, there are no other abnormal finding on examination. There is no lymphadenopathy. On otoscopic examination, you see a bulging and erythematous right tympanic membrane (see photo). Pneumatic otoscopy shows decreased TM mobility. His left tympanic membrane is normal. Which of the following will be a part of his treatment plan today? (choose all which apply)