The key reaction that led to Boyer’s proposed loose-tight-op…
Questions
The key reаctiоn thаt led tо Bоyer’s proposed loose-tight-open mechаnism for the reaction catalyzed by ATP synthase is shown below. The experiment was done in the presence of 18O labeled water with the free purified enzyme. It was the results of this experiment that led him to propose this unique mechanism of action for the synthesis of ATP by ATP synthase in the late 1950s. Briefly explain what aspect of this experiment implied that the ATP is formed by ATP synthase all by itself outside of the cell. (2 pts.) ADP + Pi + H2O18