The BioRad package insert procedure for anti-S is listed be…
Questions
The BiоRаd pаckаge insert prоcedure fоr anti-S is listed below. Review the procedure and identify the testing phase you will use in antigen typing your patient. Manual Tube Method: Place one (1) drop of Gamma-clone Anti-S (Monoclonal) or Gamma-clone Anti (Monoclonal) into a properly labeled test tube. Add one (1) drop of an approximate 2-5% suspension of the red blood cells to be tested to the test tube (from step 1 above). The red blood cells to be tested should previously have been washed at least one time and resuspended in saline. Mix the test tube contents well by gently shaking the tube and incubate the tube for five (5) to fifteen (15) minutes at room temperature. Incubating for the upper end of the time range may enhance reactivity. Centrifuge the test tube.* After centrifugation, immediately resuspend the red blood cells by gently shaking the test tube and examine for macroscopic agglutination. Negative reactions may be examined with an optical aid; however, microscopic reading is not recommended. Record the results. *Suggested centrifugation time and RCF: 15 to 30 seconds at 900-1000 xg or a time and speed, appropriate for the centrifuge used, that produces the strongest reaction of antibody with antigen-positive red blood cells, yet allows easy suspension of antigen-negative red blood cells. Stability of Reaction: Following centrifugation, the tube test should be read immediately and interpreted without delay.
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