Read the statements below and write “T” for the true and “F”…
Questions
Reаd the stаtements belоw аnd write “T” fоr the true and “F” fоr the false statements. (Mycorrhizal methods) (10 pts)a) Plant roots are stored in water in the lab before they are processed in the lab. [1]b) Roots are cleared in 50% KOH for 5 hrs in oven. [2]c) Roots are stained in 0.03% CBE for 1 hr in oven. [3]d) Roots are destained in 50% glycerol after they are stained. [4] e) Very thick and woody roots are mounted on the slides for AM quantification. [5]f) Soils are sieved through 250 um, 100 um and 45 um to extract the spores. [6]g) Soils are centrifuged at 5000 RPM for 30 min for each sample. [7]h) Total of 100 fields of the view are quantified to express mycorrhizal percent colonization. [8]i) If there are 5 vesicles under the field of the view, it should be counted 5 vesicles regardless of how many vesicles are intersected by eye piece cross hair. [9]j) Spore density can be expressed as number of spores per gram of dry soil. [10]