In the liver, the first step in glycоlysis is nоt cаtаlyzed by hexоkinаse, but by glucokinase. The Km of hexokinase is 0.1 mM while the Km for glucokinase is 5 mM. Upload an image of your answers to this question. Knowing this, under identical conditions and with the same concentration of glucose, which enzyme, hexokinase or glucokinase, would you predict to produce product at the highest reaction rate? Briefly justify your answer. Assume that both enzymes have similar Vmax values. (2 pts.) Michaelis-Menton kinetics assumes a two-step reaction process. Given the data above, which of the two steps in the process is most likely differs for the two enzymes. Briefly justify your answer. (2 pts.) The value of kcat for hexokinase is reported to by 28/sec. In the presence of 0.02 mM of the compound known as LY-2121260 it is reported to be 75/sec. Briefly explain if these values describe a difference of Km or Vmax or both between the activity of hexokinase in the presence LY-2121260 or not and what effect this compound has on the enzymatic activity of hexokinase. (2 pts.) Sketch and label a rough Lineweaver-Burke plot of initial velocity (1/Vo) as a function of substrate concentration (1/[S]) for the enzyme glucokinase relative to the enzyme hexokinase. Note: This is a relative plot (not exact) to show the difference in activity of the two enzymes compared to each other. Assume that both enzymes have similar Vmax values. (3 pts.) Sketch and label a second Lineweaver-Burke plot of initial velocity (1/Vo) as a function of substrate concentration (1/[S]) that compares hexokinase with and without an uncompetitive inhibitor. Note: This is a relative plot (not exact) to show the difference in activity of the enzyme with and without the inhibitor. (3 pts.)