Stephanie is investigating the signaling molecule RAND-4 in…

Stephanie is investigating the signaling molecule RAND-4 in mice. Not much is known about RAND-4 so far. She uses in situ hybridization to show that RAND-4 mRNA is expressed in the developing spinal column and hindlimbs of mice. Seeking to learn more about the role of RAND-4 in mouse development, she decides to design a RAND-4 “knockout” mouse that is missing both normal copies of RAND-4. What type of experiment is this?