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Which enzyme is primarily responsible for adding new deoxyribonucleotides to a growing DNA strand during replication in prokaryotes?

In prokaryotes, in which part of the cell do replication, transcription, and translation occur?

A researcher constructs a plasmid that carries a constitutively active ampicillin resistance gene. They introduce this plasmid into a population of bacterial cells. After transformation, the researcher needs to identify which cells have successfully taken up the plasmid. Question:Which type of growth medium should the researcher use to ensure that only the bacteria containing the plasmid survive, and why?

Which plasmid carries the genes necessary to facilitate its own conjugation between bacterial cells?

A researcher has a very small sample of DNA and needs to make millions of copies in order to study a specific gene. Because the original amount of DNA is insufficient for experiments like sequencing or cloning, the researcher decides to use a laboratory technique that can rapidly amplify the DNA. Question:Which technique should the researcher use to amplify the DNA, and what is the basic principle behind it?

Which of the following best defines genetic engineering?

A ________ blot is used to detect specific DNA sequences on a membrane, while a ________ blot is used to detect specific RNA sequences on a membrane. embrane.

A researcher is using genomics approaches to identify pathogens in patient samples by analyzing their DNA. While this method works well for most microbes, the researcher notices that certain infectious agents cannot be reliably detected using standard genomics techniques. Question:Which type of pathogen would genomics NOT be effective in detecting?

Which of the following enzymes is responsible for joining two sugar-phosphate backbones of DNA?

A patient is diagnosed with a bacterial infection, and the doctor prescribes a class of antibiotics that work by disrupting the enzymes responsible for maintaining DNA structure during replication. By targeting these enzymes, the drugs prevent the bacteria from properly replicating their DNA, stopping their growth. Question:Which enzymes are specifically targeted by quinolone antibiotics in bacteria?