Which technique allows one to purify proteins directly from organs (such as from gizzards)?
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A protein has a pI of 7, and is in a buffer at pH 5. What ty…
A protein has a pI of 7, and is in a buffer at pH 5. What type of column should it bind to?
The native gel below shows three proteins (A-C) run alone, a…
The native gel below shows three proteins (A-C) run alone, and then with a protein of interest (P). Which protein interacts with protein P?
In which expression system does T7 RNA polymerase enzyme tra…
In which expression system does T7 RNA polymerase enzyme transcribe the gene of interest on the plasmid into mRNA?
TARP protein (with domains A through D) was tested to determ…
TARP protein (with domains A through D) was tested to determine its ability to polymerize actin, which will then be found in the pellet after centrifugation. Which of the following is TRUE regarding TARP?
The gel below of an E. coli induction with IPTG is showing
The gel below of an E. coli induction with IPTG is showing
What pH must you use to purify a protein with a pI of 4.2 vi…
What pH must you use to purify a protein with a pI of 4.2 via anion exchange chromatography?
When the phage bacterial expression system is off,
When the phage bacterial expression system is off,
Why can’t SDS-PAGE be used as a purification technique for l…
Why can’t SDS-PAGE be used as a purification technique for large amounts of protein?
Which protein migrates the slowest in a native gel?
Which protein migrates the slowest in a native gel?