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Dr. Jones is doing research on an endangered species of leopard living wild in the African savannah. This method is a bit more dangerous than some methods and does not give him much control over his subjects. Which method of observation is he using?

A circular collimator(diaphragm) produces a round beam that is _____ inch (es) in diameter.

A small(short) object-film distance will increase magnification of the tooth image.

The definition of vertical angulation is:

Which type of genomics file is most efficient for obtaining all mutations that lead to changes in amino acid sequences?

Which of the following is considered the oldest form of biotechnology that was developed about 6000 BC?

A 78-year-old who has been admitted to the hospital with dehydration is confused and incontinent of urine. Which nursing action will be best to include in the plan of care?

Your goal is clone your favorite gene NRG1 using restriction digestion with type II restriction enzymes, followed by ligation in a pET plasmid, and transformation in E. coli competent cells.  The objective is to clone NRG1 between the NcoI and XhoI of the pET22b. This will allow NRG1 protein to be in frame with the pelB leader peptide and be transported to the E. coli periplasm (secretion pathways are cool), where NRG1 can fold properly. NRG1 can be amplified by PCR from cDNA of neuronal cells. How will you make sure that NRG1 will be ligated to the NcoI and XhoI sites of pET22b? (Alternatively, you can outline the steps necessary to clone a gene). 

Refer to the table below to answer the following questions.  You obtained some RNA sequencing data. Below is the 5′- sequence of a disease-associated transcript frame that should produce a protein.  GAGAUCCUAUGGAGGACACAGUAUGUGAGAGUAGCCA.  What is the partial amino acid sequence of the protein? 

Still keeping with your cloning of NRG1. Something terrible happening. You find that NRG1 contains an NcoI cut site right in the middle of the gene. What other methods could you use to clone NRG1 between NcoI and XhoI? Explain your procedure.