Category: Uncategorized

ALK is a transmembrane receptor tyrosine kinase (RTK). Normal expression of ALK is restricted to the brain and nervous system. In about one third of human large-cell lymphomas, a t(2;5)(p23;q35) chromosomal translocation causes fusion of ALK to nucleophosmin (NPM) that is ubiquitously expressed, generating a novel NPM-ALK fusion protein. The NPM-ALK fusion protein consists of the first 117 amino acids of the NPM protein (this part is known to be involved in protein dimerization/oligomerization), and the intracellular part of ALK. Which of the following statements is NOT true?

Which of the following statements regarding DNA helicase activity is false?

How does the base excision repair pathway recognize DNA damage?

The cancer drug Imatinib (Gleevec) _____.

Which ONE of the following statements regarding DNA polymerase I is false?

After completion of an Okazaki fragment, β clamps are released from Pol III and recycled. In order to recycle the clamps, the clamps must be unloaded from the DNA. What unloads the clamps, or do the clamps spontaneously unload themselves after completion of an Okazaki fragment? To examine this question, the following experiment was performed by the O’Donnell lab. A substrate was prepared by loading 32P-labeled β clamp onto a circular nicked double-stranded DNA, then the substrate was incubated for 0-1500 seconds with either purified Pol III (panel C), γ complex (panel B), purified δ subunit of the clamp loader (panel D), or substrate alone (panel A). Incubation reactions were then size-fractionated on an agarose gel, and the gels were subjected to autoradiography on X-ray film. In panel D, δ subunits were incubated with substrate for 5 minutes with increasing amounts of δ subunit, then fractionated on an agarose gel, and autoradiographed as in the other experiments. The upper band in the gels represents the β clamp complexed with the DNA substrate, the lower band represents free clamp.  Based on the results below, which of the components assayed has clamp unloading activity?  

All of the following statements regarding normal Ras signaling are true except ___.

The MSH2/MSH6 complex is necessary for mismatch repair because it _____

Most metastatic melanomas carry activating mutations in one of the RAF genes, BRAF. The following drugs except __ may efficiently block this aberrantly activated pathway:

Herceptin is an effective treatment against breast cancers with amplified Her2 receptor because it targets Her2 and _______.  Select all that apply.