In ligation independent cloning,
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You perform Sanger sequencing on a plasmid which came from a…
You perform Sanger sequencing on a plasmid which came from an E. coli colony. At one position you find two different overlapping peaks at 50% of the height of surrounding peaks, which are all identical to one another. What can you conclude from this?
What primers would you design to amplify the following seque…
What primers would you design to amplify the following sequence of DNA? 5’-TTGCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTAGAGTC-3’
In the gel below, the DNA in Lane 4 is approximately
In the gel below, the DNA in Lane 4 is approximately
100 micrograms/ml of DNA would give an A260 of
100 micrograms/ml of DNA would give an A260 of
You use a dot blot to analyze a specific locus. Who is homoz…
You use a dot blot to analyze a specific locus. Who is homozygous at this locus?
What is the advantage to introducing mutations into gene tha…
What is the advantage to introducing mutations into gene that is in already in a vector, rather than from a piece of linear DNA?
VNTR analyzes differences in DNA between individuals by comp…
VNTR analyzes differences in DNA between individuals by comparing
You wish to insert a gene into the vector below, grow the ce…
You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzyme(s) should you use?
The purity of a DNA sample is determined by looking at the
The purity of a DNA sample is determined by looking at the