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Imagine you wanted to use a human genomic DNA library to clone the human gene for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what order would you perform the following steps to accomplish this goal? Fall 2020_Exam 3_question 12 picture.png 

Pulse-field electrophoresis is useful for separating

Using these terms: affinity chromatography, agarose gel electrophoresis , gene imprinting, histone deacetylase, normal HPLC, polyacrylamide gel electrophoresis, reverse HPLC, size exclusion gel chromatography, southern blotting ,western blotting, 

In an indirect immunofluorescence assay, which component is fluorescently labeled?

In a single PCR cycle consisting of 30 seconds at 95C, 1 min at 55C, and 1 min at 72C, what is happening in the step run at 55C?

You have ligated a foreign DNA fragment into the EcoRI site in the multiple cloning site of pUC18. You then transform host E. coli with the ligation mixture. Some of the bacterial colonies growing on the nutrient agar plate that contains ampicillin and X-gal are white and some are blue. Bacterial colonies that are blue

ELISA is based on

A linear strand of DNA is 1,000 bp long. A recognition sequence for the restriction enzyme Eco R1 is located 300 base pairs (bp) from the 5′ end of this linear DNA molecule. Digesting this DNA molecule with Eco RI would produce _____.

A _____ consists of cloned DNA fragments for all expressed genes in a particular tissue

To separate acidic proteins from basic proteins in a crude cellular lysate you could use