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A febrile 18‑month‑old boy is diagnosed with a catheter‑confirmed UTI. He is stable, tolerating fluids, and able to take oral medication. What is the recommended antibiotic duration?

An adult female patient with a history of recurrent UTIs was diagnosed with a UTI 3 days ago and began a 5 day course of empiric trimethoprim-sulfamethoxazole (TMP-SMX). The urine was sent for culture and results are now available:  Culture results: E. Coli >100,000 CFU/mL Sensitivity Report:  Antibiotic  Sensitivity  Ampicillin R Cefuroxime S Nitrofurantoin S TMP-SMX R Ciprofloxacin S Based on the sensitivity report, what is your next step?

Questions 18-22 refer to the prompt below. To better understand the mechanism of azithromycin, researchers studied the effects of the azithromycin on the proteome of P. aeruginosa.  In order to do this they exposed multiple strains of P. aeruginosa to the azithromycin and compared the results to the same strains without antibiotic exposure. Below are example results from a 2 dimensional gel electrophoresis of a strain of P. aeruginosa exposed to azithromycin. Figure 2.A: Example protein profile for P. aeruginosa. Proteins were labeled with CyDye and separated by 2-D gel electrophoresis on 24 cm pH 4-7 linear gradient strips followed by electrophoresis on 12% SDS-polyacrylamide gels.  Proteins were visualized by scanning for CyDye fluorescence.  The position of the identified protein spots that were modulated by azithromycin are indicated with circles and named.

Is 2-D gel electrophoresis sufficient for identifying the function of the proteins on the gel? To fully answer the question be sure to address the following prompts. What can comparative proteomics using 2-D gel electrophoresis tell us about the proteome? (one sentence maximum) What technique is required to determine the function of an individual protein in the proteome? (one sentence maximum) Describe the process (including important steps) required for identifying the function of an individual protein in the proteome. (five sentences maximum)

Please access the exam below and enter your solutions/responses in the answer fields that correspond to each question. Before starting, please read the instructions on the first page of the exam paper at the link below carefully. Please complete the remote exam in Honorlock and type your answers directly into Honorlock/Canvas for submission. Submissions in any other format, including photos, scans, PDFs of handwritten work, or emailed answers, will not be accepted. Final Exam (PDF)

Describe at least two ways that diligent use of formative assessment affects the climate of learning in the classroom. 

Metabolic acidosis can be caused by which of the following?

Which NEC article addresses the installation of receptacles, cord connectors, and attachment plugs (cord caps)?

Which practice reduces caught-in or between hazards on a job site?

Which statement correctly describes a DOL apprenticeship requirement?