In restriction enzyme-based cloning, digesting DNA to produc…

In restriction enzyme-based cloning, digesting DNA to produce sticky ends rather than blunt ends is often preferred. Furthermore, the subsequent ligation of these ends requires specific biochemical interactions at the cloning sites. Which of the following accurately describes the functional advantage of sticky ends AND the correct biochemical role of the 5′-phosphate during the ligation step?

When comparing traditional column-based oligonucleotide synt…

When comparing traditional column-based oligonucleotide synthesis to microarray-based (chip) synthesis, which of the following accurately describes a primary advantage of the microarray method and a corresponding technical hurdle that must be overcome for successful gene assembly?