A PCR reaction is performed and the results are being analyz…

A PCR reaction is performed and the results are being analyzed.  The positive control has a band at the expected size, the negative template control has a band at the same size as the positive control; the contamination control has no band; the amplification control has a band of the expected size.  All of the patient samples that were run had a band the same size as the band seen in the positive and negative template control wells.  How should you interpret these results.  

DNA of interest  is extracted from a clinical sample.  The a…

DNA of interest  is extracted from a clinical sample.  The absorbance at 260 nm is 0.489 and the absorbance at 280 nm is 0.485.  Is this sample of sufficient quality for use in subsequent analysis?  To receive credit, you must show your calculation and concisely explain your assessment with regards to the extracted DNA’s purity.