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You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?

Which of the following will occur if the annealing temperature in PCR is 5 °C below the primer Tm?

Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?

In RAPD PCR,

You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?

A technique that involves eliminating genomic DNA, using reverse transcriptase to generate cDNA, and using a hot-start polymerase and SYBR Green dye is

In asymmetric PCR,

A VNTR analysis is performed on a mother, father, and child at a specific locus, shown below. Which of the following is TRUE?

You want to clone a 5000 bp piece of DNA. What is the best way to accomplish this?

DNA can be detected in an agarose gel by using each of the following EXCEPT