(2 pts) After these experiments, you realize the strategy of…

(2 pts) After these experiments, you realize the strategy of using a scaffold to deliver VEGF has limitations. You then decide to use a different strategy to deliver VEGF, which is genetically engineering cells to constitutively produce VEGF in culture. To do so, you choose a non-viral approach to deliver DNA sequences encoding VEGF into cells. What is one non-viral approach you would choose? With your chosen method, is the production of VEGF in cells transient or stable?    

Assume you inoculated 2 loopfuls of a bacterial broth cultur…

Assume you inoculated 2 loopfuls of a bacterial broth culture into each of two flasks of tryptic soy broth.  One flask contains 250 mL of broth (1/4 of a liter). The second flask has 500mL of broth (1/2 a liter).  At the end of 10 hours, you would expect to find …  (by comparison of broth tubes contain 5 mL of broth  Time would be relatively short for growing the culture.)

A uniform solid sphere of mass 1.5 kg and diameter 30.0 cm s…

A uniform solid sphere of mass 1.5 kg and diameter 30.0 cm starts from rest at A and rolls without slipping down a 35° frictionless incline that is 7.0 m long. The sphere is launched horizontally off the end of the track and lands on the ground below.Isphere=25MR2{“version”:”1.1″,”math”:”I_{sphere} = \frac{2}{5}MR^2″}  a. Calculate the linear speed of the sphere when it reaches the bottom of the incline at B. b. Determine the speed of the sphere when it lands on the floor below.