You are trying to perform a Sanger sequencing reaction on th…

You are trying to perform a Sanger sequencing reaction on the template below (Black) using the primer in red, and for nucleotides you include ONLY the following in the reaction: dATP, dTTP, dGTP, and ddCTP.       Could the following product be produced during a sequencing reaction with the above conditions? 5’-GCATGCAGAGTAGATCTAG-3’    

An E. coli cell is isolated with mutations that disrupt meth…

An E. coli cell is isolated with mutations that disrupt methylation marks from being installed on the parental DNA prior to genome replication, leading to a loss of hemimethylated DNA following replication. These mutations will most affect which pathway in the cell: