Which of the following is true regarding blunt end digestion and subsequent ligation?
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What must a plasmid contain to ensure proper replication by…
What must a plasmid contain to ensure proper replication by DNA polymerase?
Which technique was used to capture Taq-treated DNA for the…
Which technique was used to capture Taq-treated DNA for the sequencing of the human genome?
In the gel below, the DNA in Lane 3 is approximately
In the gel below, the DNA in Lane 3 is approximately
A sequencing gel has two clearly defined lanes for G and C,…
A sequencing gel has two clearly defined lanes for G and C, and then a purine and pyrimidine lane. This is likely
You wish to insert a gene into the vector below, grow the ce…
You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?
When two sticky ends join, they make ________ between the ba…
When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.
You design a PCR primer to a target sequence with an EcoRI o…
You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)
What is the Tm of the primer CGTAT?
What is the Tm of the primer CGTAT?
Which molecule allows for real-time visualization of DNA dur…
Which molecule allows for real-time visualization of DNA during gel electrophoresis?