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A sequencing gel has two clearly defined lanes for G and C, and then a purine and pyrimidine lane. This is likely

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?  

When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.

You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)

What is the Tm of the primer CGTAT?

Which molecule allows for real-time visualization of DNA during gel electrophoresis?

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “B” represent?

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “A” represent?

The purpose of screening a library is to

Which technique can detect pathogens in blood samples by using RNA polymerase to synthesize mRNA, which can be detected with a probe?