Which of the following is common to both RFLP and VNTR?
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You insert a gene into the vector below using the EcoRI and …
You insert a gene into the vector below using the EcoRI and SphI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?
Which of the following can be used to inhibit RNase activity…
Which of the following can be used to inhibit RNase activity?
When screening a library via hybridization,
When screening a library via hybridization,
What primers would you design to amplify the following seque…
What primers would you design to amplify the following sequence of DNA? 5’-ATCCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTCGGATC-3’
Which of the following is FALSE regarding the screening of a…
Which of the following is FALSE regarding the screening of a gene library?
Which is NOT used in Sanger sequencing?
Which is NOT used in Sanger sequencing?
You perform Sanger sequencing on an E. coli colony from a pl…
You perform Sanger sequencing on an E. coli colony from a plate, and actually see two sequences as shown below. What can you conclude from this? …GTGCACCGC… …GTGCTCCGC…
A mutation (TGA) was introduced into the protein-coding gene…
A mutation (TGA) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGCATGAATGACAGATTTGAC-3’
In touchdown PCR,
In touchdown PCR,