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Which technique would be BEST for verifying that a vector took up an insert?

Taq polymerase is advantageous over Klenow fragment because Taq

Why is Pfu sometimes added to a PCR mixture that already has Taq polymerase?

Which enzyme can prevent ligation between two fragments?

Which step of PCR involves a variable temperature, that can change depending on the specifics of the experiment?

Two pieces of DNA can be joined together by

Which technique can be used to amplify upstream and downstream elements from a gene by digesting it, circularizing it, and re-digesting it?

Many vectors have an f1 origin. This allows for

Which lane below represents a digestion with XbaI and EcoRI?

To purify plasmids from E. coli you can use