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Which of the following is FALSE regarding the screening of a…

Which of the following is FALSE regarding the screening of a gene library?

Published June 15, 2021
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Which is NOT used in Sanger sequencing?

Which is NOT used in Sanger sequencing?

Published June 15, 2021
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You perform Sanger sequencing on an E. coli colony from a pl…

You perform Sanger sequencing on an E. coli colony from a plate, and actually see two sequences as shown below. What can you conclude from this? …GTGCACCGC… …GTGCTCCGC…

Published June 15, 2021
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A mutation (TGA) was introduced into the protein-coding gene…

A mutation (TGA) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGCATGAATGACAGATTTGAC-3’ 

Published June 15, 2021
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In touchdown PCR,

In touchdown PCR,

Published June 15, 2021
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In the gel below, the DNA in Lane 5 is approximately

In the gel below, the DNA in Lane 5 is approximately

Published June 15, 2021
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In TA cloning,

In TA cloning,

Published June 15, 2021
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A DNA microarray is typically used for all of the following…

A DNA microarray is typically used for all of the following EXCEPT

Published June 15, 2021
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You digest a ~2000 bp plasmid with EcoRI, and run a linear m…

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “D” represent?

Published June 15, 2021
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The most commonly-used method to detect DNA on a gel is

The most commonly-used method to detect DNA on a gel is

Published June 15, 2021
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