Blog

Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?

In RAPD PCR,

You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?

A technique that involves eliminating genomic DNA, using reverse transcriptase to generate cDNA, and using a hot-start polymerase and SYBR Green dye is

In asymmetric PCR,

A VNTR analysis is performed on a mother, father, and child at a specific locus, shown below. Which of the following is TRUE?

You want to clone a 5000 bp piece of DNA. What is the best way to accomplish this?

DNA can be detected in an agarose gel by using each of the following EXCEPT

You insert a gene into the vector below using the SacI and AvrII cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

You insert a gene into the vector below using the HpaII and EcoRI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?