A primer has a Tm of 50 °C, but you attach a restriction enzyme cut site which increases it to 55 °C. Which of the following is TRUE?
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Which of the following transformation techniques provides a…
Which of the following transformation techniques provides a time constant, which indicates if transformation was successful?
You wish to optimize a PCR reaction, and perform the experim…
You wish to optimize a PCR reaction, and perform the experiment at several different temperatures (from ~40-60 °C). Which step of PCR is was modified?
Which technique involves linking primers directly to beads t…
Which technique involves linking primers directly to beads to allow for easy retrieval, analysis, and to reduce contamination?
In the pedigree below, which represents the dot blot you’d e…
In the pedigree below, which represents the dot blot you’d expect of the child?
In Maxam-Gilbert sequencing,
In Maxam-Gilbert sequencing,
Three DNA samples undergo VNTR analysis, as shown below. Arr…
Three DNA samples undergo VNTR analysis, as shown below. Arrows denote digestion sites. Which of these three individuals is represented in Lane 2 on the gel to the right?
You wish to delete an entire protein domain. What is a pract…
You wish to delete an entire protein domain. What is a practical approach to this?
Which is TRUE regarding the various polymerases?
Which is TRUE regarding the various polymerases?
Which of the following is FALSE regarding Taq and Pfu DNA po…
Which of the following is FALSE regarding Taq and Pfu DNA polymerases?