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PART E Serial Dilution III) How much liquid did you transfer to each 9ml dilution blank?         

Some species of Armadillos are known to carry leprosy, a disease caused by a bacterium known to have high lipid contents in its cell walls. a) Name a staining procedure you could perform, if you were reckless enough to wipe one of their noses, to check for leprosy. b) What color would the target bacterial cells be after this procedure? c) Explain why you would not use a different staining method on these high lipid bacterial cells? d) What coloration would you expect the bacteria to have after a gram stain? Answer each question in 1-2 sentences maximum  

It is important to add loading dye to your samples when running a gel to ensure that you can visualize the DNA after imaging with UV light.    

Choose the most correct answer: The microbial quality of food is determined by:

1. What is microbial succession? 2. Explain how the concept of microbial succession is linked to the sauerkraut experiment.

In our pGLO experiment, we saw bright glowing colonies under UV light. What ingredient was present in the media that the pGLO-transformed bacteria were plated on, to make the GFP gene be expressed?

What is the significance of each step in bacteria transformation? CaCl2 in the transformation solution Incubation on ice for 10 minutes Heat shocking for 50 seconds at 42 degrees Celsius Incubation in LB prior to plating Answer in 1-2 sentences each

In this class, we have looked at two different kinds of food preservation: milk and solid foods. Comparing the two, it can be said that the solid food preservation differs from the milk preservation as it is a controlled natural fermentation. Define this term in a sentence and describe the way(s) this is achieved in the preservation of solid food.

T cells differ from B cells by expressing –

Inflammation is one of the first signs of damage or infection. Which cells do you expect to be the most numerous in a new inflammation?