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Why does linear DNA separate based on size in an agarose gel?

In qPCR, SYBR Green is not specific to the gene of interest. What is used to account for this?

Which type of restriction enzyme digestion guarantees the directionality of your insert?

To create a transgenic plant you should use a

Which technique CANNOT be used to generate a 10,000 bp piece of DNA for cloning?

Guanidinium isothiocyanate is used in RNA purifications to

Which of the following is FALSE about the probe in qPCR?

Which lane below represents a digestion with XbaI and XhoI?

Viruses can be detected in the very early stages in patients with specific primers via

You wish to insert a protein-coding gene into the vector below, and would like it to contain a single C-terminal His-tag upon expression. What should the primers look like? 5′—EcoRI—BamHI—His-Tag—3′