Which of the following will occur if the annealing temperature in PCR is 5 °C above the primer Tm?
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Some restriction enzymes cut incorrectly in the wrong buffer…
Some restriction enzymes cut incorrectly in the wrong buffer. This is called
Which lane below represents a digestion with PstI and SmaI?
Which lane below represents a digestion with PstI and SmaI?
All of the following are used as vectors for human gene ther…
All of the following are used as vectors for human gene therapy EXCEPT
To purify DNA from an agarose gel you would perform a(n)
To purify DNA from an agarose gel you would perform a(n)
You use Sanger sequencing on a 1000 bp insert, but after ~50…
You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?
Which of the following will occur if the annealing temperatu…
Which of the following will occur if the annealing temperature in PCR is 5 °C below the primer Tm?
Who discovered Thermus aquaticus, the bacteria from Yellowst…
Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?
In RAPD PCR,
In RAPD PCR,
You want to design a protein that contains an EcoRI cut site…
You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?