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Which of the following will occur if the annealing temperatu…

Which of the following will occur if the annealing temperature in PCR is 5 °C above the primer Tm?

Published June 15, 2021
Categorized as Uncategorized

Some restriction enzymes cut incorrectly in the wrong buffer…

Some restriction enzymes cut incorrectly in the wrong buffer. This is called

Published June 15, 2021
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Which lane below represents a digestion with PstI and SmaI?

Which lane below represents a digestion with PstI and SmaI?

Published June 15, 2021
Categorized as Uncategorized

All of the following are used as vectors for human gene ther…

All of the following are used as vectors for human gene therapy EXCEPT

Published June 15, 2021
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To purify DNA from an agarose gel you would perform a(n)

To purify DNA from an agarose gel you would perform a(n)

Published June 15, 2021
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You use Sanger sequencing on a 1000 bp insert, but after ~50…

You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?

Published June 15, 2021
Categorized as Uncategorized

Which of the following will occur if the annealing temperatu…

Which of the following will occur if the annealing temperature in PCR is 5 °C below the primer Tm?

Published June 15, 2021
Categorized as Uncategorized

Who discovered Thermus aquaticus, the bacteria from Yellowst…

Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?

Published June 15, 2021
Categorized as Uncategorized

In RAPD PCR,

In RAPD PCR,

Published June 15, 2021
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You want to design a protein that contains an EcoRI cut site…

You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?

Published June 15, 2021
Categorized as Uncategorized

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