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Which technique would efficiently separate small organic molecules (like vitamins) for further use?

You express a protein in E. coli at 37 °C, lyse the cells, and perform a western blot (below). Which of the following would be an appropriate next step?

The western blot of an SDS-PAGE below shows ARD1’s role in ubiquitination of proteins. The full-length ARD1 (1-574) is shown in the first lane, with other various constructs in the following lanes. Which of the following is TRUE?

Which would be the best choice for studying protein-protein interactions or protein modifications?

What is the purpose of Tween-20 in the western blot washes?

In the SDS-PAGE gel from an affinity column below, Lane 2 shows the

From the gel below you can conclude that

Two proteins elute in fractions 30 and 40 of a size exclusion column on their own. You mix them in equal molar ratios and run them over the same column. What would you expect if they interact?

In the Ptac expression system,

Which is TRUE about N-terminal sequencing?