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In Meselson and Stahl’s experiments, bacteria were grown in…

In Meselson and Stahl’s experiments, bacteria were grown in a medium containing “heavy” nitrogen (15N) and then transferred them to a medium containing 14N for two rounds of replication.  After the DNA is isolated and centrifuged to separate by weight, which of the figures to the right would be expected if the DNA is replicated by a semiconservative mechanism?

ALK is a transmembrane receptor tyrosine kinase (RTK). Norma…

ALK is a transmembrane receptor tyrosine kinase (RTK). Normal expression of ALK is restricted to the brain and nervous system. In about one third of human large-cell lymphomas, a t(2;5)(p23;q35) chromosomal translocation causes fusion of ALK to nucleophosmin (NPM) that is ubiquitously expressed, generating a novel NPM-ALK fusion protein. The NPM-ALK fusion protein consists of the first 117 amino acids of the NPM protein (this part is known to be involved in protein dimerization/oligomerization), and the intracellular part of ALK. Which of the following statements is NOT true?

After completion of an Okazaki fragment, β clamps are releas…

After completion of an Okazaki fragment, β clamps are released from Pol III and recycled. In order to recycle the clamps, the clamps must be unloaded from the DNA. What unloads the clamps, or do the clamps spontaneously unload themselves after completion of an Okazaki fragment? To examine this question, the following experiment was performed by the O’Donnell lab. A substrate was prepared by loading 32P-labeled β clamp onto a circular nicked double-stranded DNA, then the substrate was incubated for 0-1500 seconds with either purified Pol III (panel C), γ complex (panel B), purified δ subunit of the clamp loader (panel D), or substrate alone (panel A). Incubation reactions were then size-fractionated on an agarose gel, and the gels were subjected to autoradiography on X-ray film. In panel D, δ subunits were incubated with substrate for 5 minutes with increasing amounts of δ subunit, then fractionated on an agarose gel, and autoradiographed as in the other experiments. The upper band in the gels represents the β clamp complexed with the DNA substrate, the lower band represents free clamp.  Based on the results below, which of the components assayed has clamp unloading activity?