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CRISPR/Cas9 is sometimes used to “knock-in” donor DNA to a desired locus. Select all methods that are used to increase the frequency of these events. 

Which of the following methods has the highest genomic target site versatility? i.e. it allows for the most flexible targeting of DNA sequence.

Genetic lineage tracing sometimes makes use of one or more transgenic loci that allow the the protein products (called fluorophores) expressed from them to be permanently changed. How is this usually accomplished?

CRISPR (Clustered regularly space palindromic repeats) describes a genomic region found in [blank1] that naturally [blank2].

FITT is a helpful tool for counting the calories you consume.

An alert adult patient is receiving CPAP with PS of 5 cm H2O and an FIO2 of 0.28.  The patient has a heart rate of 88 and a respiratory rate of 22. Blood gas analysis results are as follows: pH 7.43 PaCO2 35 torr PaO2  95 torr HCO3- 23 mEq/L BE -1 mEq/L Which of the following is a respiratory therapist’s most appropriate action?

Carbohydrates are an important fuel source for the brain and muscle during exercise.

Compared to aerobic metabolism, anaerobic metabolism produces a very large amount of ATP.

Proteins are considered a major energy source.

Which of the following are possible complications of performing bronchoscopy with biopsy? bronchorrhea      laryngospasm       pneumothorax    hemoptysis A yes yes   yes no B yes yes no yes C yes no yes yes D no yes yes yes