Blog

You are growing up your protein in LB/Kan broth. You pour your 20mL preculture that grew overnight into 980mL of LB/Kan broth. You place your LB/Kan broth into the incubator at 37oC and check the OD600 every 30 minutes. After your media makes it to an OD600 of 0.5, you add in 1M IPTG to achieve a final concentration of 1.0mM. How much IPTG did you add to the media in order to achieve a final concentration of 1.0mM? Please give your answer in

Some of the ATP stock from the previous question is diluted to a volume of 1 L and a concentration of 7 ug/mL. How much of the ATP stock was used? Give your answer in mL. 

You have a stock container of 5X buffer, but your experiment requires 1X buffer. If you want to make 1.5 L, how much of the 5X buffer do you need? Please give your answer in mL.

Before the 1880s, most immigrants came from _________; after the 1880s, most immigrants came from _______.

In the English Peppered moth example, which is incorrect:

How many grams of caffeine would you need in order to prepare 75 mL of a 15 µM solution of caffeine? The Mw of caffeine is 456 g/mol.

Extra space only if needed.

In ocean acidification:

Please select from the following dilutions in order to make a 1:900 dilution in the least amount of steps. Please select all that apply.

Dominic Price calls his inventory the