11.  A unicellular organism is discovered in remote lake. It…

11.  A unicellular organism is discovered in remote lake. It possesses no nucleus, haploid DNA, and an abundance of phosphatidylcholines in its cell membrane.      a) In which Domain does this organism belong:  Archaea, Bacteria, or Eukarya?  (2 pts)      b) The organism is found to be aerobic.  What does this mean?  (2 pts)      c) List the five characteristics of life, most of which this organism should exhibit.  (4 pts)      d) This organism’s F1F0-ATPase has 14 c subunits in its c collar.  Calculate the ATP yield from one of the following fuels – your choice.  Assume aerobic conditions and use of the malate-aspartate shuttle.  Do NOT do both.  (6 pts)                 myristic acid  (Myristic acid is 14:0.  Don’t forget the cost of activation.)         OR                          fructose-6-phosphate        e) The organism is found to biosynthesize glycine by the following novel pathway.  Below four of the yields arrows are blanks labeled 1 through 4; fill them in by either giving the specific enzyme class name or EC number of the enzyme employed.  For EC numbers, just fill in the given blanks; sometimes one or two digits are needed.  (6 pts – 1 ea. blank)         

12.  Below is the 5’ to 3’ sequence of DNA that codes for pa…

12.  Below is the 5’ to 3’ sequence of DNA that codes for part of a protein.          a) Use the given base sequence to construct both the mRNA sequence coded by the DNA and the protein coded by the mRNA.  You may use 1- or 3-letter code for the protein sequence.  You may construct the 3’ to 5’ DNA sequence from which the mRNA is built, but doing so is not necessary.  (6 pts – 3 pts mRNA; 3 pts protein) 5’ to 3’:  G A C C T A G C G C G T C T G G T G A G T C T C T T T G C T A T G T A T 3’ to 5’: mRNA: protein:         b) Examine the amino acid sequence you have found.  Does it code for an alpha-helix, beta-sheet, intrinsically disordered region, or loop?  Point out two features of the amino acid sequence that support your conclusion.  (6 pts)