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Which of the following is NOT usually found in a PCR mixture?

Which type of PCR involves using multiple sets of primers to amplify several pieces of DNA in the same reaction mixture?

Which of the following is true regarding blunt end digestion and subsequent ligation?

What must a plasmid contain to ensure proper replication by DNA polymerase?

Which technique was used to capture Taq-treated DNA for the sequencing of the human genome?

In the gel below, the DNA in Lane 3 is approximately

A sequencing gel has two clearly defined lanes for G and C, and then a purine and pyrimidine lane. This is likely

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?  

When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.

You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)