(8 pts)  Describe your testing and analysis methods step by…

(8 pts)  Describe your testing and analysis methods step by step with as much detail as possible, addressing the required topics PLUS choose at least 2 more from the choices listed.  Be sure to use the words listed as you describe each topic to get full credit.

(12 pts) You and your team then decide to pick an existing b…

(12 pts) You and your team then decide to pick an existing bioreactor design as a reference to design your own device to grow tissue constructs.  (not cells alone)  Please list the name of the basic models shown below PLUS 1 advantage and 1 disadvantage of each bioreactor type listed—be sure to list with the letters A, B, C, D and type advantage and disadvantage to clearly answer the question.   

(2 pts) Your client also mentions that the bioreactor will b…

(2 pts) Your client also mentions that the bioreactor will be placed in a culture incubator to grow engineered ligaments for a long period of time and should be reusable after each experiment. To achieve these ends, name 1 requirement the material you choose to fabricate the bioreactor should meet for each of the underlined conditions.  (2 material requirements total)

(2 pts) You have performed a number of experiments in which…

(2 pts) You have performed a number of experiments in which different doses of VEGF are added to culture by pipetting, to induce iPSC differentiation, but the results are not satisfactory. You realize that the poor results may be caused by the concentration of VEGF in culture, which is mainly due to how VEGF is administered into the culture. Please describe a VEGF concentration curve over time in the culture, which is resulted from manual injection of the molecule.  Describe the shape of the curve over time in relation to the therapeutic range and the name generally given to this type of concentration curve.  (A graph without a curve is provided below for your reference.)