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What is the sequence of the strand of DNA on the right side of this figure (the strand enclosed in the red box)?  

Where would primers to initiate DNA replication be found on the following piece of DNA? Hint: Only the parental DNA strand is shown. The position refers to the general area.

What would be observed in the event of loss of heterozygosity?

In PCR-SSOP, the HLA allele is identified based on which of the following?

This test is used to determine if the mismatch repair system is functioning normally.

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue stain. What is the interpretation of this assay?

A woman requires a kidney transplant. Five members of her family would like to be tested to determine if they are eligible donors. Technologists use sequence-specific oligonucleotide probe hybridization methods to perform HLA typing. Which of the following anticoagulants is preferred when collecting blood from the woman and her family members?

In HLA nomenclature, the letter N indicates what type of allele?

A 57-year-old female sought medical attention for chronic fatigue, weight loss, and bone pain. Tissue from an abnormal mass was sent to the molecular diagnostics laboratory. Results from flow cytometry indicated the presence of a kappa-restricted B-cell population consistent with lymphoma. To aid in the diagnosis, the sample was further tested for immunoglobulin heavy-chain gene rearrangements by PCR. After electrophoresis of the PCR product, a smear pattern similar to lane 2 in Figure 13-22 was observed.   What is the interpretation of the gel pattern with the smear? 

Which of the following molecular-based methods can be used to perform HLA typing?