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What is the role of Protospacer Adjacent Motif (PAM) in the CRISPR/Cas9 system?

The next two questions are related to the following issue. Mr A presents with a sore red tongue, fatigue and difficulty concentrating. A blood test is requested to investigate the cause of the patient’s symptoms.

What is a potential complication of untreated gonorrhoea for this patient?

If Miss C’s hypothyroidism is not well controlled, what potential complication is she at risk of in pregnancy?

What is Mr A’s strongest risk factor for type 2 diabetes?

Which of Miss C’s presenting complaints most strongly suggests an alternative diagnosis to urinary tract infection?

Which of the following is the most appropriate advice to provide to Mr A relating to the use and storage of Ozempic? https://www.medicines.org.uk/emc/product/9748

Miss C has been prescribed prednisolone 5mg daily for the last 6 months for a chronic inflammatory condition, and now has cellulitus (skin) infection requiring IV antibiotics. Select the most appropriate regimen. 

Mr A informs you that he participates in Ramadan. Utilising the following resource, what is the most appropriate advice to provide to Mr A regarding his medications during this period? https://www.england.nhs.uk/midlands/wp-content/uploads/sites/46/2020/11/SWBCCG-Summary-Guidance-Diabetes-Ramadan-V1.pdf

Miss C usually takes her contraceptive pill with good adherence every day without any problems, but she realised she missed her pill yesterday as she was feeling quite anxious about her appointment at the sexual health clinic and had left the house at a different time to normal. She is on day 4 of her pill pack and last had sex 3 days ago without a condom. She confirms she has not missed any other doses of her Mercilon tablets. What advice would be most appropriate regarding contraceptive cover?