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This test is used to determine if the mismatch repair system is functioning normally.

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue stain. What is the interpretation of this assay?

A woman requires a kidney transplant. Five members of her family would like to be tested to determine if they are eligible donors. Technologists use sequence-specific oligonucleotide probe hybridization methods to perform HLA typing. Which of the following anticoagulants is preferred when collecting blood from the woman and her family members?

In HLA nomenclature, the letter N indicates what type of allele?

A 57-year-old female sought medical attention for chronic fatigue, weight loss, and bone pain. Tissue from an abnormal mass was sent to the molecular diagnostics laboratory. Results from flow cytometry indicated the presence of a kappa-restricted B-cell population consistent with lymphoma. To aid in the diagnosis, the sample was further tested for immunoglobulin heavy-chain gene rearrangements by PCR. After electrophoresis of the PCR product, a smear pattern similar to lane 2 in Figure 13-22 was observed.   What is the interpretation of the gel pattern with the smear? 

Which of the following molecular-based methods can be used to perform HLA typing?

Which of the following denotes a null allele in the HLA locus?

In PCR-SSOP methods for HLA typing, the primers used to amplify the region are complementary to sequences present in which exon of the class II genes?

T-cell receptor gene rearrangements were analyzed by PCR using forward and reverse primers complementary to the Vg1 and Jg1 regions, respectively, of the T-cell receptor gene region. The products were separated by polyacrylamide gel electrophoresis and bands visualized using ethidium bromide. The lane corresponding to the patient had a sharp band. All controls were acceptable. How is this result interpreted?

A patient is assessed for B-cell clonality by examining the immunoglobulin heavy chain gene rearrangements using PCR with primers complementary to the variable and joining regions of the immunoglobulin heavy chain gene. When the products are resolved by gel electrophoresis, the patient lane has a smear and does not have a sharp band. Controls were all acceptable. This result is interpreted as which of the following?