Virtual Lab Q: What is the purpose of including known TNF1 a…

Virtual Lab Q: What is the purpose of including known TNF1 and TNF2 DNA samples in the loading sequence? A:  The purpose of including known TNF1 and TNF2 DNA samples in the loading sequence is to serve as positive controls. This helps verify that the PCR and gel electrophoresis processes are functioning correctly by ensuring that the known DNA sequences are properly amplified and detected.  

Virtual Lab Q: What is the purpose of using a running buffer…

Virtual Lab Q: What is the purpose of using a running buffer instead of deionized water when running a gel? A: Running buffer provides ions that facilitate the conduction of electric current through the gel, which is essential for separating DNA or proteins during electrophoresis.  

Each person inherits two copies of any given gene from their…

Each person inherits two copies of any given gene from their parents. Those who inherit two copies of the same gene variants (variants are called alleles) are known as homozygous individuals (the prefix “homo-” means “the same”). Those who inherit two different alleles of the gene are known as heterozygous individuals (the prefix “hetero- “means “different”). Whether an individual is homo- or heterozygous is their “zygosity.” Determine and record the zygosity of samples 1, 2, and 3, from the simulation, in the table below.   DNA Sample 1 DNA Sample 2 DNA Sample 3 Zygosity (homo/hetero) [DNA1] [DNA2] [DNA3] Allele (s) contained (TNF1 and/or TNF2) [DNA4] [DNA5] [DNA6]