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2. Which of the following is the most logical order of a typical gene cloning experiment?   I – Recombination of gene into cloning vector (sub-cloning) II – Cloning and selection III – Restriction digestion of gene and cloning vector DNA IV – Transformation to host cell V – Isolation of gene and cloning vector DNA through agarose gel electrophoresis

6. What amino acid sequence will be generated, based on the following mRNA codon sequence? 5′ AUG-UCU-UCG-UUA-UCC-UUG 3′ (Use the Genetic Code!) 

6. Which enzymes were used to produce the cut DNA molecule in the figure below?

4. Which of the following components does NOT form part of the transcription complex at a eukaryotic promoter?

9. Place the steps in a cycle of PCR (Polymerase Chain Reaction) in the correct order.    1.Annealing—Cool mix to allow primers to form hydrogen bonds with ends of target sequence 2.Extension—DNA polymerase adds free nucleotides to the 3′ end of each primer 3.Denaturation—Heat briefly to near boiling temp to separate DNA strands

1. Which of the following molecules bridges DNA to protein? 

8. Erwin Chargaff’s observations of DNA lead to the complimentary base pairing rules.  Based on what he proposed, if you find an organism w/ 14% Thymine in their DNA, what would you expect the other base amounts to be?

5. Which of the following is NOT a modification made to eukaryotic mRNA after it is created in transcription before it leaves the nucleus to be translated on a ribosome (ie. mRNA processing)?

3. Achondroplasia, a common form of dwarfism, is caused by a Dominant allele.  If 2 Heterozygotes for this trait were to mate, what % of their offspring will be normal height? 

5.  In the late 1950s, Meselson and Stahl grew bacteria in a medium containing heavy nitrogen (15N) and then transferred them to a medium containing lighter nitrogen (14N). Which of the results in figure below would be expected after one round of DNA replication in the presence of 14N?