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You conduct an ELISA sample to test the presence of antibodies in a patient sample.  After conducting the entire experiment, you suddenly realize that you did not add any substrate (antigen) to the wells in the initial step.  Under such circumstances, the development of a colored reaction in the patient sample (positive result) would indicate a ——-

Cells that have NOT undergone transformation with pGFP should be killed after plating  on LB-AMP.  

In the following figure, the 1:32 dilution of the sample will have less antibodies than the 1:2 dilution (assuming that the dilutions are done correctly)

In a transformation experiment, nutrient Agar plates containing the antibiotic Ampicillin were used in the experiment for what purpose?

The following gel shows the results of a crime scene investigation.  Lane 1 shows a DNA sample that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so it’s the criminal’s blood sample that produced the DNA bands in lane 1). Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime.  Which suspect (#2 through #7) is most likely to have committed the crime?

In the following picture, the bands are orange in color because of ————— binding to ————–.

After transformation of E. coli with pGFP , cells that take up the plasmid exhibit fluorescence upon exposure to UV because of expression of ————protein from the plasmid DNA.  

While performing a quadrant streak (also called the streak plate method) with a bacterial sample, the purpose of sterilizing the loop between each quadrant is:

The presence of more than one kind of colony on an agar plate indicates that the original sample used to streak the plate consists of a mix of different types of bacteria.

The following picture taken from a rotting potato? What conclusions can you make from the clear zones seen on the plate?