You are running an enzyme assay that uses the co-enzyme NAD. Its activity is measured in terms of NADH produced. You have obtained the following data: Total volume of assay is 3.0 mL Sample volume is 0.5 mL Absorbance change 7 minutes is 0.350 The molecular extinction coefficient of NADH is 6.22 x 10-3 L/μmol-1/cm-1 and the light path is 1 cm. Calculate the enzyme activity in IU/L.
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You are given a series of 6 tubes. In the first tube is 4.5…
You are given a series of 6 tubes. In the first tube is 4.5 mL of saline. In each of the following tubes is 6 mL of saline. 0.5 mL of serum is added to the first tube and 2 mL of this is then transferred to each of the following tubes. The final 2 mL is discarded. The total (substance) dilution in tube #3 is:
You are making up a standard curve for an albumin assay. The…
You are making up a standard curve for an albumin assay. The standard values that are needed are: 1.0 g/dL; 2.0 g/dL; 4.0 g/dL; 6.0 g/dL; 8.0 g/dL; and 10.0 g/dL. You have at your disposal 100 mL of a 30 g/dL stock solution. What is the volume of stock and dilution needed to make 5 mL of the 2.0 g/dL standard?
0.5 mL of serum is used in an ALT test. The change in absorb…
0.5 mL of serum is used in an ALT test. The change in absorbance is measured at 340 nm using a 2 mL reaction mixture. The initial absorbance reading was 0.63. The reading after 3 minutes was 0.41. What is the ALT in IU?
Convert 750 microliters to milliliters.
Convert 750 microliters to milliliters.
Which is NOT an interference for the urine reagent strip tes…
Which is NOT an interference for the urine reagent strip test for leukocyte esterase?
A quantitative D-dimer immunoassay test is performed on a pa…
A quantitative D-dimer immunoassay test is performed on a patient using an automated instrument. The value is 1160 ng/mL (110-240 ng/mL). Which of the following best fits this result?
After a meal, a urinalysis sample of an otherwise healthy in…
After a meal, a urinalysis sample of an otherwise healthy individual appeared cloudy but had normal chemical and microscopic results. What would be the cause of the increased turbidity in this sample?
A CLS spills a bottle of concentrated sulfuric acid and slip…
A CLS spills a bottle of concentrated sulfuric acid and slips in the fluid exposing the lower length of her body to the burning fluid. What should be the best thing another CLS could do?
Consider these total protein electrophoresis results: ALB 40…
Consider these total protein electrophoresis results: ALB 40 (53-65%) αlpha1 2.3 (2.5-5%) αlpha2 39 (7-13%) beta 7 (8-14%) Gamma 11 (12-22%) What is the most likely interpretation?