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Which fixative is useful for a post fixative of electron microscopy as it will preserve phospholipids?

When observing the results of a Von Kossa stain microscopically, it is noted that the calcium salts are staining a yellow to yellow brown color instead of the expected black. What is a plausible explanation for these results?

A pathologist requests both the Alcian blue, pH 2.5 and a Periodic Acid Schiff stain to be done on a tissue sample. These stains can be used to differentiate:

Formalin pigment is identified in a section of spleen. This pigmentation can be removed by treating the sections with:

A pathologist suspects that a patient has Alzheimer’s disease, what stain would be helpful in making this determination?

A quality control record indicates the paraffin melting point is between 52 C to 54 C degrees. Ideally the temperature for the tissue flotation bath should be established at:

A section of skin shows an abundant amount of melanin which is obscurring the cellular detail.  Which technique can be used to bleach melanin from tissues?

Microscopic evaluation of a Masson trichrome reveals that the connective tissue is staining a faded blue instead of the expected crisp bright blue color. What can be done to prevent this from happening again?

What most likely caused the reaction shown here of a section of kidney stained with PAS?  10(2).jpg 

The most suitable method of determining the end point of decalcification for a bone specimen fixed in Zenker’s would be….?