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List the basic steps for DNA extraction.

The detergent [1] is commonly used in the lysis buffer to disrupt cellular membranes.

What is the main reason for including a final extension step at the end of the PCR cycles?

In DNA sequencing, how is a Q score value of 30 interpreted in terms of base calling accuracy?

DNA concentration is calculated based on absorbance at 280 nm using the NanoDrop.

The [1] system of the NanoDrop allows for the measurement of highly concentrated samples without dilutions.

Ethanol is used during DNA precipitation because it makes the DNA more soluble in water.

What should be avoided during the drying step of bead clean-up to prevent yield loss?

Describe the workflow of using the NanoDrop to measure DNA concentration.

In 16S sequencing, [1] and [2] diversity are used to assess the variation within and between samples.