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RNA Polymerase was shown biochemically to bind tightly to the following 50 nucleotide piece of double-stranded DNA from the E. coli genome, indicating that it likely contains a basal prokaryotic promoter.  The -10 promoter recognition sequence is underlined. Star indicates the nucleotide pair that occurs at the +1 transcription start site. Given the double-stranded sequence below, answer the following questions:                        Please watch your spelling!    a) Based on your analysis of this promoter region, which strand, top or bottom, will be the template strand? Template will be [tmpl] strand   b) Based on your analysis of this promoter region, in which direction, left or right, will transcription proceed?Transcription will move to the [drc]   c) Write the first eight nucleotides that will make up the beginning of the mRNA made from this promoter:The first 8 nucleotides will be: 5’ [sequence]  

 What is the role of the Sigma (s) factor in E. coli transcription?

Which dog is more floofy? 

Which of the following modifications tend to increase transcription?

The large ribosomal RNAs are transcribed by

This enzyme is required for replication of the complete prokaryotic genome: DNA primase

Indicate whether the following statements about eukaryotic transcription factors (TF) are true or false The recognition sequences are mostly located on the minor groove of the DNA

The activities of homeodomain transcription factors in controlling development are based on different homeodomain transcription factors bind to different DNA sequences.

Which of the following ‘omes can be predicted as indicated?

The structure of DNA has the following properties The purine bases pair with each other, and the pyrimidine bases pair with each other