You are running an enzyme assay that uses the co-enzyme NAD….

You are running an enzyme assay that uses the co-enzyme NAD. Its activity is measured in terms of NADH produced. You have obtained the following data: Total volume of assay is 3.0 mL Sample volume is 0.5 mL Absorbance change 7 minutes is 0.350 The molecular extinction coefficient of NADH is 6.22 x 10-3 L/μmol-1/cm-1 and the light path is 1 cm. Calculate the enzyme activity in IU/L.

You are given a series of 6 tubes. In the first tube is 4.5…

You are given a series of 6 tubes. In the first tube is 4.5 mL of saline. In each of the following tubes is 6 mL of saline. 0.5 mL of serum is added to the first tube and 2 mL of this is then transferred to each of the following tubes. The final 2 mL is discarded. The total (substance) dilution in tube #3 is:

You are making up a standard curve for an albumin assay. The…

You are making up a standard curve for an albumin assay. The standard values that are needed are: 1.0 g/dL; 2.0 g/dL; 4.0 g/dL; 6.0 g/dL; 8.0 g/dL; and 10.0 g/dL. You have at your disposal 100 mL of a 30 g/dL stock solution. What is the volume of stock and dilution needed to make 5 mL of the 2.0 g/dL standard?